Description

The Sylvester Comprehensive Cancer Center is looking for an Associate Scientist to join the newly established Cancer Proteomic Shared Resources, to apply state of the art LC-MS based methods and techniques for quantitative proteomics.

As an Associate Scientist, you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer (Orbitrap Ascend or Astral Zoom) as well as performing detailed biochemical sample processing techniques. In addition, you will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology. 

The individual will be:

• Primary responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument or Primary responsibility for the use of a new Astral Zoom instrument for proteomic applications.
• Will manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks.
• Prepare protein samples from a wide range of specimen (e.g., cells, tissues, biofluids, and SDS Page gels)
• Implement of both bottom-up & top-down proteomic approaches.
• Responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and TMT mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation).
• Responsible for structural proteomics (e.g., cross-linking, pull-down experiments).
• Will conduct Single cell proteomics analysis.
• Intact protein analysis & MW determination.
• Use of proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis.
• Responsible for operation, maintenance, and troubleshooting of Mass Spectrometers and other laboratory equipment as needed.
• Assesses experimental results and troubleshoot methods and instruments to optimize research protocols.
• Collaborates efficiently with technicians, graduate students, post-docs, PIs, the laboratory director & colleagues.

Requirements

Knowledge, Skills and Attitudes:

• 5 years of related experience with mass spectrometry instruments which includes 2 years of experience in proteomics mass spectrometry applications.
• Hands-on experience operating and/or maintaining LC-MS instruments and supporting equipment 
• Possess strong protein biochemistry skills and attention to detail
• Demonstrates exceptional motivation to pursue cancer research
• Ability to work both independently and collaborative in a team environment
• Excellent analytical,  problem solving, and organizational skills.
• Excellent written and oral communication skills
• Established track record of publications and demonstrated ability to complete projects.
• Capability to learn complex tasks with instruction and to use experience to perform a variety of new techniques.
   

Preference will be given.

Expertise in one or more of the following technical areas: quantitative proteomics, single cell proteomic analysis, global proteome profiling.
Previous hands-on operation of Thermo Tribrid Orbitrap LC-MS Systems (e.g., Fusion, Eclipse, Ascend, Astral or Astral Zoom).